《植物生理学报》 2013, 49(12): 1363-1367

春石斛优良品种‘森禾2006’组培快繁体系的建立

贾梦雪¹, 徐瑾¹, 叶香娟², 刘芊¹, 王喆², 刘燕^1,*

¹北京林业大学园林学院, 国家花卉工程技术研究中心, 北京100083; ²浙江森禾种业股份有限公司, 杭州310012

通信作者：刘燕；E-mail: chbly@sohu.com；Tel: 010-62336062

摘　要：

本文研究了春石斛‘森禾2006’的拟原球茎(PLBs)诱导、增殖、分化及壮苗和生根, 建立了其组培快繁体系。结果表明: ‘森禾2006’ PLBs最适诱导培养基为1/2MS+TDZ 0.5 mg·L^-1+水解酪蛋白2.0 g·L^-1; PLBs增殖培养基为1/2MS+水解酪蛋白2.0 g·L^-1; PLBs分化培养基为1/2MS+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1; 壮苗生根培养基为MS+IBA 0.5 mg·L^-1+NAA 0.1 mg·L^-1+ 香蕉泥100.0 g·L^-1。

关键词：春石斛; 拟原球茎(PLBs); 组培快繁; 种苗

收稿：2013-08-06   修定：2013-09-18

资助：国家“十二五”支撑计划课题(2011BAD12B02-01)

Establishment of Rapid Propagation System for Elite Variety of Dendrobium nobile Lindl.‘Senhe 2006’by Tissue Culture

JIA Meng-Xue¹, XU Jin¹, YE Xiang-Juan², LIU Qian¹, WANG Zhe², LIU Yan^1,*

¹National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China; ²Zhejiang Senhe Seed Company Limited, Hangzhou 310012, China

Corresponding author: LIU Yan; E-mail: chbly@sohu.com; Tel: 010-62336062

Abstract:

To achieve the mass-scale multiplication of Dendrobium nobile ‘Senhe 2006’, the optimal media for the induction, multiplication and differentiation of protocorm-like bodies (PLBs), strengthening and rooting were studied and the rapid propagation system by tissue culture was established. The results showed that 1/2MS+TDZ 0.5 mg·L^-1+casein hydrolysate 2.0 g·L^-1 was optimal for PLB induction. 1/2MS+casein hydrolysate 2.0 g·L^-1 was the most suitable for PLB multiplication. 1/2MS+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1 was the most efficient for PLB differentiation. The optimum medium for strengthening and rooting was MS+IBA 0.5 mg·L^-1 +NAA 0.1 mg·L^-1+banana homogenate 100.0 g·L^-1.

Key words: Dendrobium nobile; protocorm-like bodies (PLBs); tissue culture and rapid propagation; plantlets